Stim validating

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The cells are subsequently fixed, permeabilized, and reacted with a dye-labeled azide, catalyzed by copper.A covalent bond is formed between the dye and the incorporated nucleotide, and the fluorescent signal is then measured by flow cytometry.(4) Specific proliferating cell populations can be visualized by the addition of cell-specific antibodies.Controls in this laboratory and most clinical laboratories are healthy adults.

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Cell viability can also be measured within the same assay without requiring additional cell manipulation or specimen.It should be noted that without obtaining formal pediatric reference values it remains a possibility that the response in infants and children can be underestimated.However, the practical challenges of generating a pediatric range for this assay necessitate comparison of pediatric data with adult reference values or controls.Circadian variations in circulating T-cell counts have been shown to be negatively correlated with plasma cortisol concentration.In fact, cortisol and catecholamine concentrations control distribution and, therefore, numbers of naive versus effector CD4 and CD8 T cells.

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